Protoparmeliopsis muralis is a common lichen that occur on different substrata and is distributed worldwide. It is always fertile and develops numerous apothecia. Several different Trebouxia species associate with this lichen-forming fungus and such flexibility allows successful colonization of new habitats and survival of the lichen. To better understand the biology of P. muralis the sequencing of genomic DNA was performed. Genomic DNA was isolated from mycelium of P. muralis and sequencing was performed using a whole-genome shotgun strategy with Illumina HiSeq 2000 to about 79-fold coverage using Macrogen (Korea) sequencing service (www.macrogen.com). All sequences were assembled into 757 scaffolds containing 2575 contigs, with a genome size of 44.7 Mb and 43% GC content. Subsequent gene prediction analysis was performed using AUGUSTUS software. This presentation will focus on several genes that might be important for life strategy of P. muralis.
The research was partially supported by Marie Curie European Reintegration Grant no. 239343 within the 7th FP and statutory funds from the University of Gda?sk.