Nitric oxide (NO) is a critical signaling molecule with vast biological impacts in mammals (cardiovascular, neurological, immunological systems, etc.) and in plants (growth, development, and defense against stresses).Administering exogenous NO has been challenging due to the instability and poor bioavailability of NO donors.Recently, the combination of nanomaterials with NO donors has emerged as a promising strategy to tailor the spatio-temporal release of therapeutic amounts of NO. Nanoscience offers smart approaches for this on-demand delivery.Although promising, this strategy has been explored more extensively in biomedical research and less in agricultural applications.The current review summarizes the impact of NO and its donors in biomedical research and agriculture, highlighting recent progress in combining nanotechnology and NO donors. The goal is to discuss the advantages, challenges, and drawbacks of this strategy to inspire the translation of these innovations into clinical and practical settings.